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KMID : 0811720090130040301
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Korean Journal of Physiology & Pharmacology
2009 Volume.13 No. 4 p.301 ~ p.307
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DHA and EPA Down-regulate COX-2 Expression through Suppression of NF-?B Activity in LPS-treated Human Umbilical Vein Endothelial Cells
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Lee Soon-Ae
Park Ji-Kwon
Paik Won-Young
Shin Jeong-Kyu
Lee Jong-Hak
Kim Hye-Jung
Chang Ki-Churl
Choi Won-Jun
Baek Jong-Chul
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Abstract
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Inflammatory processes of vascular endothelial cells play a key role in the development ofatherosclerosis. We determined the anti-inflammatory effects and mechanisms of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on LPS-treated human umbilical vein endothelial cells (HUVECs) to evaluate their cardioprotective potential. Cells were pretreated with DHA, EPA, or troglitazone prior to activation with LPS. Expression of COX-2, prostaglandin E2 (PGE2) and IL-6 production, and NF-?B activity were measured by Western blot, ELISA, and luciferase activity, respectively. Results showed that EPA, DHA, or troglitazone significantly reduced COX-2 expression, NF-?B luciferase activity, and PGE2 and IL-6 production in a dose-dependent fashion. Interestingly, low doses (10?M) of DHA and EPA, but not troglitozone, significantly increased the activity of NF-?B in resting HUVECs. Our study suggests that while DHA, EPA, and troglitazone may be protective on HUVECs under inflammatory conditions in a dose-dependent manner. However there may be some negative effects when the concentrations are abnormally low, even in normal endothelium.
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KEYWORD
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DHA, EPA, Cyclooxygenase-2, Nuclear factor-?B, Endothelium
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